STED Microscopy ·
A Doughnut of Light
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.com · a red ring switches molecules off · only the dark hole still glows
Spot
200 nm
STED
0 × Isat
Abbe
200 nm
Phase
DIFFRACTION-LIMITED
Excitation beam
STED doughnut — dark centre
Fluorescence that reaches the detector
Super-resolved image
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